The successful candidate will join an innovative Antibody Discovery and Engineering Department, with a strong record of success in developing novel antibody therapeutics in the field of immuno-oncology. The successful candidate will support mammalian antibody expression platforms and cell-line engineering to facilitate discovery activities. We are looking for a motivated and talented scientist with a deep understanding and hands-on experience in molecular biology, cell line engineering as well as antibody expression and purification to advance the next wave of novel therapeutic concepts.

SPECIFIC RESPONSIBILITIES:

• Transient protein expression/production- Antibodies (mg to gram-scale) and antigens- in different host cell lines (CHO, HEK) in flasks to support candidate selection


• Design and cloning of DNA constructs, perform DNA preparations


• Participate in stable pool generation to produce candidates to enable in vitro, in vivo and non-human primate studies. Perform production in bioreactors.


• Perform FACS staining for antigen detection, cancer cell line characterization


• Data analysis


• Purification of antigens and antibodies (Ni-NTA, Protein A, CEX) and characterization using different analytical methods (e.g., SDS-PAGE, SE-HPLC, IEF)


• Management of media stocks for all Antibody Discovery and Engineering department Present and discuss data in English in both informal and formal settings


• Maintain detailed and organized documentation


• Participate in day-to-day laboratory management


• Perform other relevant duties as required


• Collaborate across the matrix to support portfolio development


• Comply with HSE guidelines

EDUCATION AND DESIRED EXPERIENCE

• BSc/MSc in Biotechnology, Biochemistry or related discipline (e.g. cell biology/engineering) with demonstrated analytical skills is required


• Experience in the following: molecular biology, antibody or protein expression and characterization desired


• Experience in stable CHO pool development based on transposons is preferred


• Strong expertise in mammalian cell culture (small to large scale) and transfection methods is desired


• Strong experience with PCR, mutagenesis, DNA handling software, and bioinformatics tools


• Knowledge of antibody analytical methods is a plus


• Excellent time management and organizational skills


• Demonstrated ability to work in a multidisciplinary biologics discovery team


• Ability to manage multiple priorities and align with internal and external stakeholders around these priorities


• Excellent written and oral communication skills are necessary


• Professional level in English, both written and verbal.

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